Interlaboratory Comparison of Fetal Male DNA Detection from Common Maternal Plasma Samples by Real-Time PCR
نویسندگان
چکیده
منابع مشابه
Interlaboratory comparison of fetal male DNA detection from common maternal plasma samples by real-time PCR.
BACKGROUND Analysis of fetal DNA from maternal plasma by PCR offers great potential for noninvasive prenatal genetic diagnosis. To further evaluate this potential, we developed and validated a standard protocol to determine whether fetal DNA sequences could be reproducibly amplified and measured across multiple laboratories in a common set of specimens. METHODS Each of five participating cent...
متن کاملOptimized real-time quantitative PCR measurement of male fetal DNA in maternal plasma.
BACKGROUND Circulating fetal DNA (cfDNA) in maternal plasma has been measured to investigate its possible relationship with pregnancy-related disorders, including fetal trisomy 21 and preeclampsia. The circulating concentrations of single-copy fetal genes, however, are close to the detection limits of PCR methods. METHODS We optimized a protocol for the real-time quantitative PCR amplificatio...
متن کاملDetection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
متن کاملMaldi-tof mass spectrometry compared with real-time PCR for detection of fetal cell-free DNA in maternal plasma.
To the Editor: Matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently emerged as a new platform for highly sensitive and accurate analysis of DNA, especially cell-free DNA (cf-DNA) (1–3). In this context MALDI-TOF MS has been shown to permit the detection of paternally inherited single nucleotide base variations from fetal cf-DNA in maternal pl...
متن کاملchimeric external control to quantify cell free dna in plasma samples by real time pcr
background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2004
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2003.024380